Composite

Part:BBa_K2587012

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)


P_RBS_luxR_T

This part contains LuxR (BBa_K2587003:https://parts.igem.org/Part:BBa_K2587003) as a complete transcriptional unit with a constitutive promoter, RBS and Terminator. LuxR is a component of the quorum sensing system and is used as a regulator, forming a complex with the quorum sensing molecule and binding to the promoter Plux which induces synthesis of the target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 70
    Illegal BsaI.rc site found at 701


[edit]
Categories
Parameters
None